The railway bridge Estakáda Sluncová in Prague is part of the new railway connection between Prague’s main station and Prague Holešovice station. The structure is a continuous beam with total length of 323.8 m, consists of eight spans and follows a strongly curved ground view. In the course of the prevailing investigation an extensive dynamic monitoring campaign was undertaken in order to examine the Estakáda Sluncová’s initial condition. Within the scope of the present assessment the live loads’ dynamic effect on the load bearing capacity and on the operability was evaluated and recommendations for the further service life were made. Special attention was paid to the analyses of the internal pre-stressing of the primary load bearing structure (main girders in the longitudinal direction).